Friday, January 8, 2010

Gene coding question [biology]?

The base sequence of the gene coding for a short polypeptide is:


CTACGCTAGGCGATTGACT


What would be the base sequnce of the mRNA transcribed from this gene?Gene coding question [biology]?
For every DNA strand, there is a complementary strand. These two strands are joined together through the base pairs (A - C - G - T).


The bases join in the following way: A joins with T, and C joins with G.


So the complementary DNA strand's base sequence would be:


GATGCGATCCGCTAACTGA.


Then, when mRNA forms it replaces the complementary DNA strand following the same rules of base pairing, except the T is replaced by U, because mRNA lacks T and has U instead.


So, you get:


GAUGCGAUCCGCUAACUCA.


Hope you got it ;)Gene coding question [biology]?
Simple when its T then use A when A then mRna will have U





GAUGCGAUCCGCUAACUGU
assuming this is template DNA, then the gene will be transcribed to RNA. (ATGC, AUGC)





CTACGCTAGGCGATTAGACT (DNA coding strand 5' to 3')





TCGTATCGAATAGCCGAGTC (DNA template 3' to 5')





CGACGCUAGGCGAUUAGACU (mRNA 5' to 3')





mRNA is transcribed from the DNA template, not the DNA coding strand.





it really depends on what you mean by the ';base sequence of the gene coding for the polypeptide.'; Do you mean the coding strand, read in the 5' to 3' direction, like I think you do? If so, then the mRNA transcript answer I gave is correct becuase mRNA is transcribed from the template strand.
For every DNA base sequence we have their opposites purine or pyrimidine i.e. A-T,C-G then


CTACGCTAGGCGATTGACT


GATGCGATCCGCTAACTGA


But for RNA it will be


CUACGCUAGGCGAUUGACU


GAUGCGAUCCGCUAACUGA


Because in RNA thymine is replaced by uracil.
OOH! I love these types of questions!!!!


Now, here's the background information:


For every DNA base sequence, there is an 'opposite'. The opposites are:


A-T, C-G. So, for this sample, which you gave, it would be:


CTACGCTAGGCGATTGACT


GATGCGATCCGCTAACTGA





BUT!! When talking in RNA code, you replace all Ts with Us. SO!


GATGCGATCCGCTAACTGA


would be:


GAUGCGAUCCGCUAACUGA





I hope that answers your question!!!
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